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KMID : 0366119770050030153
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Korean Journal of Applied Microbiology & Bioengineering
1977 Volume.5 No. 3 p.153 ~ p.158
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Studies on the Proteolytic Enzyme of Mold
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Abstract
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These experiments were conducted to investigate the condition of the production and the heat resistance of the acid protease by Rhixopus japonicus S-62. The results obtained were as follows :
1) The optimum concentrations of sucrose, yeast, ammonium chloride and sodium phosphate monobasic added to the wheat bran medium in the acid protease production were 0.5%, 2.0%, 0.4 %, and 0.4%, respectively.
2) KH₂PO₄ and NaH₂PO₄ were the most effective as the heat resistant agents.
3) When the enzyme solutions added with KH₂PO₄ and NaH₂PO₄ to the concentration of 2 % were heated for 10 min, at 50℃, their residual activities were 100%, respectively.
4) The heat resistant effects of KH₂PO₄ and NaH₂PO₄ were not observed almost above 55℃.
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